Clinical and Experimental Reproductive Medicine

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Original Article
Korean J Fertil Steril. 1990;17(1):87-91.
Development of Chemiluminescence Immunoassay for Progesterone in Serum
KS Lee1, BH Suh1, JH Lee1, JB Kim2
1Infertility Clinic, Department f Obstetrics and Gynecology, Collage of Medicine, Kyung Hee University
2Department of Animal Products Science, Science, Collage of Animal Husbandry, Kun-Kuk University
Abstract
Development of a solid-phase chemiluminescence immunoassay for the detection of progesterone in serum extract was described. The chemiluminescence immunoassay was establised utilizing anti-progesterone monoclonal antibody that coated on polystyrene tubes and progesterone-ABEI conjugate as tracer. The light yield generated from antibody bound conjugate was counted on clinilumat luminometer by oxidation with microperoxidase and peroxide. The chemiluminescence immunoassay was high specific and accurate and detects as little as 3.9ng/ml of progesterone. The intra-assay CV ranged from 6% to 11.5% and inter-assay CV ranged from 13.6% to 18.7%. This assay system was good correlated with conventional kit radioimmunoassay system (r=0.98).

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