Clinical and Experimental Reproductive Medicine

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Original Article
Korean J Fertil Steril. 1990;17(1):57-63.
Study on Pregnancy and Improvement of Fertilization rate by Micromanipulation (Partial Zona Dissection (PZD) and Micro-Insemination by Sperm Transfer(MIST) in human IVF-ET
Ho-Joon Lee1, Kyoo-Wan Choi1, Jong-Yung Jun1, Jong-Min Park1, Hyuck-Chan Kwon1, Moon-Kyoo Kim2
1IVF Research Laboratory, Department of Obstetrics and Gynecology, Cheil General Hospital
2Department of Biology, College of Natural Science, Hanyang University
Abstract
The purpose of this study is to improve fertilization rate in IVF-ET program of patients with male infertility used micromanipulation technique, partial zona dissection (PZD) or micro-insemination by sperm transfer (MIST). The results were as follows 1. The fertilization rate of non-micromanipulated oocytes and micromanipulated (PZD) oocytes were 12.5% (n=2) and 42.2% (n=19), respectively, and showed significant differences between two groups (p<0.05). 2. The fertilization rate of micromanipulated (MIST) oocytes was 30% (n=27). 3. The damage rate of Group 1 (PZD) and Group 2 (MIST) were 15.7% (3/19) and 29.6% (8/27), respectively. 4. One pregnancy resulted following replacement of micromanipulated (MIST) embryos in 4 patients.

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